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根據(jù)一份報(bào)告，比一根人類(lèi)頭發(fā)還細(xì)的微針可以在皮膚中釋放有效劑量的流感疫苗。Richard Compans及其同事用流感疫苗包裹了不銹鋼微針，并把它們插入了小鼠的皮膚中幾分鐘。在注射了這種疫苗的兩周之后，接受微針免疫的組表現(xiàn)出了與通過(guò)皮下注射針頭注射疫苗的小鼠類(lèi)似的健壯的免疫應(yīng)答。 這組作者在注射疫苗的一個(gè)月之后為5組對(duì)照組和疫苗組小鼠（每組6只）注射了高劑量的流感病毒，結(jié)果發(fā)現(xiàn)所有對(duì)照組的小鼠都死于流感，而接種疫苗組的所有小鼠——無(wú)論是通過(guò)微針注射還是皮下注射針頭注射——都活了下來(lái)。兩種疫苗組的小鼠都表現(xiàn)出了幾乎相同的對(duì)流感病毒的免疫應(yīng)答。這組作者得出結(jié)論說(shuō)，微針釋放的疫苗再加上更加容易的接種可能讓這種技術(shù)成為傳統(tǒng)疫苗注射方法的一個(gè)有吸引力的替代方案。 推薦原始出處： PNAS，doi: 10.1073/pnas.0812652106，Qiyun Zhu，Richard W. Compans Immunization by vaccine-coated microneedle arrays protects against lethal influenza virus challenge Qiyun Zhua,1, Vladimir G. Zarnitsynb,1, Ling Yea,1, Zhiyuan Wena, Yulong Gaoa, Lei Pana, Ioanna Skountzoua, Harvinder S. Gilla, Mark R. Prausnitzb,2, Chinglai Yanga,2 and Richard W. Compansa,2 Influenza prophylaxis would benefit from a simple method to administer influenza vaccine into skin without the need for hypodermic needles. In this study, solid metal microneedle arrays (MNs) were investigated as a system for cutaneous vaccine delivery using influenza virus antigen. The MNs with 5 monument-shaped microneedles per array were produced and coated with inactivated influenza virus A/PR/8/34 (IIV). As much as 10 μg of viral proteins could be coated onto an array of 5 microneedles, and the coated IIV was delivered into skin at high efficiency within minutes. The coated MNs were used to immunize mice in comparison with conventional intramuscular injection at the same dose. Analysis of immune responses showed that a single immunization with IIV-coated MNs induced strong antibody responses against influenza virus, with significant levels of hemagglutination inhibition activities (>1:40), which were comparable to those induced by conventional intramuscular immunization. Moreover, mice immunized by a single dose of IIV coated on MNs were effectively protected against lethal challenge by a high dose of mouse-adapted influenza virus A/PR/8/34. These results show that MNs are highly effective as a simple method of vaccine delivery to elicit protective immune responses against virus infection. (責(zé)任編輯：Doctor001) |
